目的 探讨11 种重楼属药用植物叶中主要次生代谢产物积累的差异。方法 采用超高效液相色谱法(UPLC)测定11 种重楼属药用植物叶中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷的含量,建立其超高效液相色谱法图谱,并用单因素方差分析、色谱法分析对其叶中主要次生代谢产物积累差异进行研究。结果 11 种重楼属药用植物叶中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量(P＜0.01)具有极显著差异,只有在阔瓣重楼、五指莲、黑籽重楼、长柱重楼和滇重楼叶中同时检测到重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ;11 种重楼属药用植物叶超高效液相色谱法图谱差异较大,主要次生代谢产物种类也存在较大差异,其中阔瓣重楼、五指莲、黑籽重楼、长柱重楼和滇重楼叶超高效液相色谱法图谱相似度均在 0.902 以上,有 16 个共有峰,主要次生代谢产物种类差异较小。结论 11 种重楼属药用植物叶合成重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ等主要次生代谢产物能力及种类存在较大差异,这可能是重楼属药用植物根茎中主要次生代谢产物含量及种类差异较大的主要原因。
Abstract
OBJECTIVE To investigate the differences of the accumulation of the main secondary metabolites in the leaves of 11 kinds of Paris L. medicinal plants. METHODS The contents of the main secondary metabolites, polyphyllinⅠ, Ⅱ, Ⅵ, and Ⅶ in the leaves of 11 kinds of Paris L. medicinal plants were determined by UPLC, and the UPLC fingerprints were established. The accumulation of the main secondary metabolites was evaluated by one-way ANOVA and chromatographic analysis. RESULTS There was significant difference(P<0.01)in the contents of polyphyllin Ⅰ, Ⅱ, Ⅵ, and Ⅶ in the leaves of 11 kinds of Paris L. medicinal plants, and polyphyllin Ⅰ, Ⅱ, Ⅵ, Ⅶ were simutaneously detected only in var. yunnanensis-1, P. axialis, P. thibetica, P. forrestii and var. yunnanensis; there was significant difference in the UPLC chromatograms of 11 kinds of Paris L. medicinal plants, but the similarities among var. yunnanensis-1, P. axialis, P. thibetica, P. forrestii and var. yunnanensis all reached 0.902 with 16 common peaks, indicating smaller difference in their main secondary metabolites. CONCLUSION There is significant difference in the abilities of the 11 kinds of Paris L.medicinal plants to accumulate polyphyllin Ⅰ, Ⅱ, Ⅵ, and Ⅶ , which may be the main reason that there are significant differences in the contents and classes of the major secondary metabolites of Paris L. roots.
关键词
重楼属植物 /
超高效液相色谱法 /
次生代谢产物
{{custom_keyword}} /
Key words
Paris L. /
UPLC /
secondary metabolite
{{custom_keyword}} /
中图分类号：
R282
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch. P(2015) VoI Ⅰ(中国药典2015年版. 一部)[S]. 2015:260.
[2] HUANG X X, GAO W Y, MAN S L, et al. Advances in studies on saponins in plants of Paris L. and their biosynthetic approach [J]. Chin Tradit Herb Drugs(中草药), 2009, 40(3):483-489.
[3] WU S S, GAO W Y, DUAN H Q, et al. Advances in studies on chemical constituents and pharmacological activities of Rhizoma Paridis [J]. Chin Tradit Herb Drugs(中草药), 2004, 35(3):344-347.
[4] ZHOU L, ZHOU N, ZHANG H Z, et al. Determination of four saponins in Paris L. polyphylla var. Yunnanensis from different habitats by HPLC [J]. West China J Pharm Sci (华西药学杂志), 2009, 24(5):521-523.
[5] LI H, SU B, ZHANG Z Y, et al. An assessment on the rarely medical Paris L. plants in China with exploring the future development of its plantation [J]. J West China Forest Sci (西部林业科学), 2015, 44(3):1-15.
[6] FENG L L, ZHANG L, LI H F, et al. Metabolism rule of secondary metabolites, steroidal saponins active ingredients in Paris L. polyphylla var. yunnanensis Franch [J]. Chin Pharm J(中国药学杂志), 2015, 50(8):664-670.
[7] GAO W W, TONG J M, GUO S L. Advances in studies on ecological functions of secondary metabolites [J]. Chin Pharm J(中国药学杂志), 2006, 41(13):961-964.
[8] LIANG Y Y, LIU Z, GAO W Y, et al. Determination of nine steroidal saponins in Paris L. polyphylla from different areas of Guizhou province by HPLC[J]. China J Chin Mater Med(中国中药杂志), 2012, 37(15):2309-2312.
[9] ZHOU A C, RUN L D, HUANG Y, et al. Chemical evaluation of germplasm resources of Paris L. Polyphylla Smith Var. Chinensis (Franch. ) Hara in Zhejiang Province[J]. Chin J Mod Appl Pharm(中国现代应用药学), 2015, 32(9):1065-1069.
[10] XIAO C H, ZHOU T, JIANG W K, et al. Dynamic accumulation of biomass and secondary metabolites of Pesudostellaria heterophylla in Guizhou province [J]. Chin Pharm J(中国药学杂志), 2013, 48(16):1346-1351.
[11] ZHAO T Z, WANG B Q, MA Q, et al. Study on best harvesting time of Paris L. polyphylla var.yunnanensis [J]. Chin Wild Plant Res(中国野生植物资源), 2014, 33(5):61-66.
[12] WANG Y H, NIU H M, ZHANG Z Y, et al. Medicinal values and their chemical bases of Paris L. [J]. China J Chin Mater Med(中国中药杂志), 2015, 40(5):833-839.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家自然科学基金资助项目(81360616);云南省教育厅基金重点资助项目(2013Z153)
{{custom_fund}}
PDF(847 KB)
